Improved genetically modified Escherichia coli strain for prescreening antineoplastic agents

Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli

Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-based cellulose; however, commercial production of BC has remained a challenge, primarily due to the strain properties of cellulose-producing bacteria. Herein, we developed a functional and stable BC production system in genetically modified (GM) Escherichia coli by recombinant expression of both the BC sy...

Construction and characterization of an Escherichia coli strain genetically engineered for Ni(II) bioaccumulation.

An Escherichia coli strain that accumulated Ni(II) was constructed by introducing the nixA gene (coding for a nickel transport system) from Helicobacter pylori into JM109 cells that expressed a glutathione S-transferase-pea metallothionein fusion protein. The resulting strain accumulated 15 micromol of Ni(II) per g (dry weight) from a 10 microM Ni(II) solution, four times the level taken up by ...

Antineoplastic Agents

Microwave improved Escherichia coli transformation.

AIMS The calcium chloride chemical transformation of Escherichia coli is still the most widly used cloning method in small laboratories. Therefore, any practicable improvement in its transformation efficiency seems to be of general interest. METHODS AND RESULTS We found that giving calcium chloride competent cells a 1 min microwave pulse at the lowest power setting (180 W), instead of the cla...

Genetically modified plants for improved trace element nutrition.

Deficiencies of iron and zinc are common worldwide. Various strategies have been used to combat these deficiencies including supplementation, food fortification and modification of food preparation and processing methods. A new possible strategy is to use biotechnology to improve trace element nutrition. Genetic engineering can be used in several ways; the most obvious is to increase the trace ...